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KMID : 0545120090190111464
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 11 p.1464 ~ p.1469
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Development of TaqMan Probe-Based Real-Time PCR Method for erm(A),erm(B), and erm(C), Rapid Detection of Macrolide-LincosamideStreptogramin B Resistance Genes, from Clinical Isolates-
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Jung Jae-Hyuk
Yoon Eun-Jeong
Choi Eung-Chil
Choi Sung-Sook
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Abstract
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To achieve more accurate and rapid detection of macrolidelincosamide- streptogramin B resistance genes, erm(A), erm(B), and erm(C), we developed a TaqMan probe-based real-time PCR (Q-PCR) method and compared it with conventional PCR (C-PCR), which is the most widely using erm gene identification method. The detection limit of Q-PCR was 5 fg of genomic DNA or 5-8 CFU of bacterial cells of Staphylococcus aureus. The utilization of Q-PCR might shorten the time to erm detection from 3-4 h to about 50 min. These data indicated that Q-PCR assay appears to be not only highly sensitive and specific, but also the most rapid diagnostic method. Therefore, the appropriate application of the Q-PCR assay will permit rapid and accurate identification of erm genes from clinical and other samples.
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KEYWORD
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TaqMan probe-based real-time PCR, erm gene, conventional PCR, Staphylococcus aureus
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